共和国勋章获得者袁隆平:"我不能躺功劳簿上睡大觉"

毕业论文 题 目 浅谈幼儿园自制玩教具的开发与应用 专业名称 学 前 教 育 1目 录一、自制玩教具的意义…………………………………………………………………（4）二、自制玩教具的作用…………………………………………………………………（4）（一）从幼儿的角度看……………………………………………………………（4）（二）从教师的角度看……………………………………………………………（5）（三）从家长的角度看……………………………………………………………（5）三、自制玩具设计原则…………………………………………………………………（5）（一）教育性………………………………………………………………………（5）（二）科学性………………………………………………………………………（5）（三）趣味性………………………………………………………………………（5）（四）创新性………………………………………………………………………（6）（五）简易性………………………………………………………………………（6）（六）安全性………………………………………………………………………（6）四、自制玩教具的材料及方法…………………………………………………………（6）（一）自然类材2料…………………………………………………………………（6）（二）纸制品、印刷类材料………………………………………………………（6）（三）布、线类材料………………………………………………………………（7）（四）塑料类材料…………………………………………………………………（7）参考文献 ………………………………………………………………………………（8）谢词 ……………………………………………………………………………………（8）浅谈幼儿园自制玩教具的开发与应用 摘要：利用各种废旧生活材料或自然材料制作的玩具既能节能减排废物利用又能达到因材施教的目的，是一种全新的玩具观念。自制教玩具的根本目的是为了能让幼儿在自制过程中发展自己各个方面的能力，让幼儿在充分的活动中得以满足，获得3知识，提高技能。一个成功的自制玩教具，必备的因素有合适的材料、巧妙的设计方法以及研究的教育设计策略和可行的教育措施。同时自制的玩教具的设计要能充分的发挥教育的优势，要根据幼儿发展需要和教育需求加以巧妙的设计，已达到有效的开展教育活动，以促进幼儿教育的发展。关键词：玩教具；自制；设计；开发Research on the Exploitation and Utilization of Homemade Toys and Teaching Aids of KindergartenAbstract: Nowadays, using various kinds of waste materials from daily life and the nature to make toys and teaching aids has become a new toy-making concept, which is internationally popularized. The purpose of making homemade toys and teaching aids is to develop children’s comprehensive skills in the process, satisfy them by sufficient activities and to gain knowledge and skills accordingly. Appropriate materials, ingenious design 4methods, rigorous strategy of educational design and workable educational measures are the indispensible factors for making an ideal homemade toy. Basing on the needs of children’s development and education, the homemade toys and teaching aids design should give full play to educational features and make full use of the educational factors to undertake educational activities, promoting children’s development to a better extent.Key words: Toys and teaching aids; Homemade; Design; Explore幼儿教育是基础教育的有机组成部分，幼儿教育的现状直接影响到未来人材资源素质，幼儿教育应受到全社会高度重视和关注。然而与此同时幼儿园的玩教具成为了幼儿教育中重要的学习资源，也对幼儿的身心发展和认知发展具有重要的作用。因为幼儿对玩教具材料的操纵活动，是幼儿感知和发现问题的途径，可以丰富幼儿的感性经验，能形成多种操纵性学习方式，能开展创造性学习活动，同时也是幼儿创新意识、实践能力的培养途径。一、自制玩教具的意义幼儿园自制玩教具，是一种教学或辅助教学的用品，它是教师根据教育需要和幼儿发展需求，对各种自然资源和材料，进行收集、分类、加工、改造、组合，重新进行玩具教育因素设计后的产物。是一种创造性的活动，由于材料的形状、用途的不固定和玩教具玩法的不受限制。这样启发性较大，联想的范围较大，探索余地较宽，容易引起幼儿的好奇心和探索行为。从改5革教学方法的角度看，它会比一般的教具更具有教育的针对性，更能体现幼儿的发展需要。这样的特点既发挥了师幼的主动性、创作性，又提高了幼儿活动的兴趣，便于幼儿的身心发展，这对幼儿园开展园本课程和特色校园的文化建设起到了重要的作用。二、自制玩教具的作用（一）从幼儿的角度看第一，幼儿身心发展的特点决定了他们不能通过长时间静坐听讲，需要以观看教师演示的方式来学习。 第二，幼儿在使用玩教具的过程中，遇到困难需要解决时，要通过不同的实验反复解决问题，这个决定问题的过程就锻炼了幼儿小制作活动的基本能力。在反复操作中，也可以培养幼儿的良好个性，而且可以增加求知欲和增强自信心。对幼儿的身心发展都有较好的发展和重要的作用。第三，教师在指导幼儿自制玩教具的过程中，引导幼儿收集资料，可以使幼儿辨认、分类、准备和了解废旧材料的回收使用价值。 第四，自制玩教具的过程可以培养幼儿的合作能力。幼儿制作玩教具是在教师和家长的配合下完成的，教师或家长便成为了幼儿的合作伙伴。这样幼儿通过交流合作，就会学着谦让、谅解、友善和团结互助。这样可以帮助幼儿在其他活动中更好的与伙伴交往与合作。（二）从教师的角度看 1.提高教师的专业水平。教师在让幼儿制作玩教具前要做多种准备活动，这样就要求教师应了解各种玩具和研究玩具等，课下不断的充实自己，更新自己的知识体系，然后根据自己所带班级幼儿的发展水平制作出适合的玩教具，这个制作的过程就是教师提高自我专业水平的过程。2.增进师生之间的感情。自制玩教具是教师了解当前幼儿各方面的发展状况的过程。在制作玩教具的过程中，通过教师传授制作方法、经验和共同制作，成为幼儿的指导者和合作伙伴，便于更好的与幼儿沟通，也让幼儿更多的了解教师，增进师生之间的感情。（三）从家长的角度看1.家长和教师的交流合作。自制玩教具对家长来说是家园同步。幼儿在幼儿园6的自制材料很多都是由幼儿家长带来的，这有利于家长了解幼儿园的教学活动，了解游戏对幼儿的重要意义。2. 增进亲子之间的感情。自制玩教具就给家长提供了一个与幼儿沟通的桥梁，在制作的过程中通过互相的探讨、研究、交流，家长开始了解幼儿的想法，鼓励幼儿积极思考，能够自己独立地解决困难。家长在引导幼儿的过程中应多鼓励幼儿，要让幼儿体会到与父母在一起做玩具的乐趣，从而增进亲子之间感情。三、自制玩教具的设计原则（一）教育性教师可以通过自制玩教具可以鼓励幼儿积极主动地参与活动，这样有益于幼儿身心健康和全面发展。教师在制作前要根据课程教材考虑自制玩教具适宜的“用途”，把“做”和“用”相结合才能发挥自制教玩具的真实价值。（二）科学性教师在设计构思自制玩教具时，应注意知识，概念与原理的正确性。符合幼儿身心发展的特点和水平。这样设计出的自制玩教具才会科学合理，适合幼儿的成长与发展。（三）趣味性学习的第一步是兴趣，这要求自制玩教具要符合幼儿的审美情趣，来源于幼儿的生活，能激发幼儿的活动兴趣，这样幼儿才能积极配合，有助于教学过程的进展。（四）创新性自制教玩具是为了弥补成品玩具的不足而设计的，单纯的模仿只会让自制玩教具变成一种摆设。那就要求自制玩教具构思要新颖，外形要独特，这样能激发幼儿的想象和创造。（五）简易性教师就应注意就地取材，成本低廉。以最低的成本制作成最适合教学的玩教具。而且制作方法应简单，不能浪费过多的精力，这样也会耽误其他的正常工作。至于使用方面，应考虑幼儿的年龄特点，制作方便、可操作的实用性玩教具。7（六）安全性由于幼儿在日常生活中与教玩具接触机会很多，可能会使幼儿受到伤害，还可能成为传播疾病的媒介。所以一定要符合安全标准和卫生要求。四、自制玩教具的材料及方法自制玩教具最重要的就是选材，那就要求教师有充分的想象力和创造力把生活中的“废垃圾”变成人见人爱的玩教具。选材虽然重要，但是堆了好多材料后，教师就应区分这 Inhibitors, Agonists, Screening Librarieswww.MedChemExpress.comSafety Data SheetRevision Date:Oct.-03-2018Print Date: Oct.-03-20181. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name : RAD51 Inhibitor B02Catalog No. : HY-101462CAS No. : 1290541-46-61.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses : Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany: MedChemExpress USATel: 609-228-6898Fax: 609-228-5909E-mail: sales@medchemexpress.com1.4 Emergency telephone numberEmergency Phone #: 609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms: B02Formula: C22H17N3OMolecular Weight: 339.39CAS No. : 1290541-46-64. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelidswith fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.Page 1 of 5 www.MedChemExpress.comInhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnelto safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces andequipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaustventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersPage 2 of 5 www.MedChemExpress.comComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controlsKeep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance Light yellow to yellow (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas) No data availableUpper/lower flammability or explosive limitsNo data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsPage 3 of 5 www.MedChemExpress.comNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible orconfirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmedcarcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmedcarcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmedcarcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.Page 4 of 5 www.MedChemExpress.com12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis)reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductiveharm.16. OTHER INFORMATIONCopyright 2018 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport tobe all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It mustonly be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safeuse of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling orfrom contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@MedChemExpress.comAddress: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USAPage 5 of 5 www.MedChemExpress.com䀝ࠤ䀝产品；防水涂料着重发展前景看好的聚氨酯、丙烯酸酯类防水涂料；密封材料仍重点发展硅酮、聚氨酯、丙烯酸酯密封膏，尽快开发防水保温一体材料；刚性防水材料、渗透结晶型防水材料、金属屋面材料、沥青油毡瓦、水泥瓦、土工材料应有一定的发展。要重视地下防水材料的开发和使用，从品种、质量和数量上满足市政建设和基础设施建设的需要。控制材料生产和施工对环境的污染和对人身健康的危害，发展绿色防水材料及施工机具。其他材料则随行就市，但要坚持扶优汰劣，坚决抵制和严厉打击假冒伪劣产品，提高防水材料整体水平。还要大力完善材料配套和施工技术，研制和供应各种材料使用的施工机具，铺设方法要多样化，大力提倡机械固定法和松铺压顶法，重视发展倒置式屋面和种植屋面。然而，建筑防水工程未来的发展有几个问题也必须尽快解决；1.市场的规范化； 2.防水材料档次的提升；3.应用技术的大力开发；4.建筑防水管理体制的改进；5. 实行防水工程质量保证期制度只有以上问题解决了，我国建筑防水工程才有可能得到进一步发展！四、结束语 建筑防水技术意义非凡，是建筑界亘古至今永恒不衰的研究话题。为求防水技术更加精湛，人类苦苦寻找防水工程遇到难题的解决方法，因此，无论古代还是现代，人类基于安全问题对防水工程总是提出各种近乎苛刻的要求，呼吁建筑界对建筑质量的绝对保证，7防水技术恰恰满足了人们的要求。目前，我国的防水技术虽然得到很大的发展，但是面对未来的各种建筑新问题，这些技术就仿若杯水车薪了，因此，我们必须尽快将该项技术提升到日程表上，努力攻坚，力争更上层楼。致谢：能够完成这篇论文，我首先要感谢的就是我的指导老师×××教授。这篇论文从创意、提出研究思路和写作提纲，到最后的完稿，均离不开×××的悉心指导。在整个过程中，××× 老师给我了多方面的极其珍贵的教诲，没有他的指导，我是不可能按期完成毕业论文的。同时，在我写作过程中，我还得到许多人的关心、支持和帮助。感谢所有的朋友们。参考文献：[1] 《中华人民共和国建筑法》[2] 《建设工程质量管理条件》规定[3] 建设部（1991 ）370 号文《关于治理屋面渗漏的若干规定》[4]《《关于印关的通知》 （建标[2001]87号）[5] 建设部（1991 ）837 号文《关于提高防水工程质量的若干规定》要求[6] 》 《地下工程防水技术规范》[7] 古代建筑文献《周礼．考工记》煦т煦比 73.09% 2.2 流动资金 万元 3204.43 2.2.1 流动资金占比 26.91% 3 收入 万元 22749.00 4 总成本 万元 17429.16 5 利润总额 万元 5319.84 6 净利润 万元 3989.88 7 所得税 万元 1.08 8 增值税 万元 733.77 9 税金及附加 万元 219.34 10 纳税总额 万元 2283.07 11 利税总额 万元 6272.95 12 投资利润率 44.67% 13 投资利税率 52.68% 14 投资回报率 33.51% 15 回收期 年 4.48 16 设备数量 台（套） 97 17 年用电量 千瓦时 970126.82 18 年用水量 立方米 14771.27 19 总能耗 吨标准煤 120.49 20 节能率 21.27% 21 节能量 吨标准煤 32.03 22 员工数量 人 428 ℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡℡RecilisibCat. No.: HY-101625CAS No.: 334969-03-8Molecular Formula: C₁₆H₁₃ClO₄SMolecular Weight: 336.79Target: Akt; PI3KPathway: PI3K/Akt/mTORStorage: Powder -20°C 3 years4°C 2 yearsIn solvent -80°C 6 months-20°C 1 monthSolvent PI3KIn Vitro Recilisib Sodium (up to 50 µM) shows a normal distribution of cells throughout the cell cycle, with a slight reduction in the number of cells in S-phase at 50 µM. Continuous exposure of Recilisib Sodium (100 µM) does not result in cell death. Recilisib Sodium does not inhibit the ability of human bone marrow to form colonies in methylcellulose at either timepoint. Recilisib Sodium treatment does not inhibit the colony forming potential of human bone marrow cells. Recilisib Sodium provides dose dependent protection of human bone marrow cells at all three doses of IR. Recilisib Sodium activates the phosphorylation of AKT and GSK3α/β in HFL cells. Recilisib Sodium increases PI3K activity in HFL-1 cells and murine bone marrow cells in response to radiation exposure. Recilisib Sodium treatment in combination with radiation alters the MAPK signaling pathway[1].Recilisib Sodium (500 mg/kg) significantly increases the rate of recovery and differentiation of primitive bone marrow In VivoProduct Data Sheet Inhibitors•Agonists•Screening Librarieswww.MedChemExpress.com1myeloid progenitor cells in mice. Recilisib Sodium in combination with radiation reduces CFU numbers in mice, but the Recilisib Sodium-treated mice consistently retain a capability to form differentiated colonies. Recilisib Sodium treated mice have a progenitor cell population that is never completely depleted by radiation exposure[1].PROTOCOLKinase Assay [1] PI3-kinase assays are performed using exponentially growing HFL-1 or freshly harvested murine bone marrow cells that are treated with increasing concentrations of Recilisib Sodium for 2 hours and then irradiated with 10 Gy IR. These cells are then returned to the incubator for 2 to 24 hours and lysed in HEPES pH 7.5 lysis buffer. PI-3K is immunoprecipitated using an anti-PI3 Kinas polyclonal antibody for 2 hours at 4°C. Protein A/G PLUS-Agarose is incubated with immunoprecipitates for 8-16 hours at 4°C and the resulting immunoprecipitates washed with twice HEPES pH 7.5 lysis buffer and once with the kinase buffer (20 mM Tris pH 7.5, 1mM EGTA, 10mM MgCl2, 2 mM DTT, 0.01% NP-40). L-α-Phosphatidylinositol (12.5 mM) and ATP (10 µM) are added to the kinase buffer (60 µL per sample) and incubated at 30°C for 30 minutes. The reaction is stopped by addition of 100 µL of 1N HCl and extracted by addition of 200 µL CHCl3/CH3OH (1:1). The extracted samples are vortexed, centrifuged and the lower organic phases containing phospholipids are dried at 27°C for 2 hours. The dried samples are resuspended in 10 µL of PI-4-P standard (0.5 mL CHCl3, 0.5 mL CH3OH, 2.5 µL HCl) and spotted on TLC plates (VWR). The spotted plate is subjected to thin layer chromatography in CHCl3/CH3OH/NH4OH (40:40:15). The TLC plate is dried and subjected to autoradiography.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Cell Assay [1] For cytotoxicity assays, cells (1.0×105 cells/mL HPGM) are treated with various concentrations of Recilisib Sodium or vehicle without radiation treatment for 2 or 24 hours. The cells are washed and plated into methocult using gridded dishes. The total number of colony forming units (CFUs) is determined 14 days post-plating by microscopic observation using an Olympus IMT-2 microscope.MCE has not independently confirmed the accuracy of these m