RepSox-DataSheet-MedChemExpress.pdf
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1、RepSoxCat. No.: HY-13012CAS No.: 446859-33-2Molecular Formula: CHNMolecular Weight: 287.32Target: TGF- ReceptorPathway: TGF-beta/SmadStorage: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 monthSolvent NaCl, 100 mM; MgCl2, 5 mM; MnCl2, 5 mM; and DTT, 10 mM). The enzyme is preincubated w
2、ith different concentrations of RepSox (0.1% DMSO final concentration in the test) for 10 min at 37C. The reaction is then initiated by the addition of 3 M ATP (0.5 Ci -33P-ATP). After 15 min at 37C, phosphorylation is stopped by the addition of SDSPAGE sample buffer (50 mM Tris-HCl, pH 6.9, 2.5% gl
3、ycerol, 1% SDS, and 5% -mercaptoethanol). The samples are boiled for 5 min at 95C and run on a 12% SDSPAGE. Dried gels are exposed to a phosphor screen overnight. ALK5 autophosphorylation is quantified using a Storm imaging system1.MCE has not independently confirmed the accuracy of these methods. T
4、hey are for reference only.Cell Assay 1 To test anti-TGF- activity of compounds, HepG2 cells are seeded in 96 well microplates at a concentration of 35000 cells per well in 200 L of serum-containing medium. The microplates are then placed for 24 h in a cell incubator at 37C, 5% CO2 atm. RepSox disso
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- RepSox DataSheet MedChemExpress