冬凌草甲素诱导多发性骨髓瘤U266细胞、RPMI8226细胞增殖和凋亡实验研究.pdf
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1、1692 结果。ApoEs4通过调控IL-6水平,使AD患者血脑屏障通透性增高,并可直接导致tau蛋白过度磷酸化,加重了 AD 患者大脑的病理改变13。TNF-是 AD 的另一种促炎细胞因子,在炎症反应过程中的细胞因子级联启动和调节中发挥核心作用14;TNF-通过上调分泌酶的产量和分泌酶活性从而加重AD患者大脑中的A病理,同时,A可通过激活转录因子直接刺激小胶质细胞产生 TNF,从而进一步加重炎症反应15。在动物实验中发现,神经元与ApoE4阳性胶质细胞共培养可明显提高TNF分泌水平,并显著降低神经元活力,而神经元与ApoE基因敲除的胶质细胞共同培养时的活力更强,分泌的TNF-水平也更低16
2、。以上研究均可支持本研究结果,说明了炎症因子水平可能加重了AD患者的病理改变,这一改变可能由 ApoE基因调控,且ApoEs4等位基因的影响更为显著。4结论综上所述,ApoEs4 基因的存在加重了AD患者的认知障碍,这一过程可能是通过炎症因子发挥作用。本研究仍存在一定局限性,本研究的样本量较小,且尚未进行长期随访,未来仍需要进一步扩大样本量,并进行纵向研究,评估炎症因子以及 ApoE基因对于 AD 患者认知障碍及炎症水平的长期影响。参考文献1任汝静,殷鹏,王志会,等中国阿尔茨海默病报告2 0 2 1J诊断学理论与实践,2 0 2 1,2 0(4):317-337.2 Scheltens P,D
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12、增殖和凋亡实验研究詹其林祝龙2 金玮韵黄菊方婷婷潘显阳吴福红李骏(1上海市公共卫生临床中心血液科上海2 0 0 0 93;2 上海市第六人民医院金山分院血液肿瘤科上海2 0 1599)【摘要】目的探究冬凌草甲素对多发性骨髓瘤(MM)U266细胞、RPMI8226细胞增殖和凋亡的影响。方法通过细胞培养和处理方法,采用0、10、2 0、30、40、50 mol/L的冬凌草甲素处理MM相关U266细胞、RPMI8226细胞48 h。采用MTT法测定U266细胞、RPMI8226细胞的增殖情况,采用AnnexinV-FITC/PI染色试剂盒检测U266细胞、RP-MI8226细胞凋亡情况,并采用蛋白质
13、印迹法测定Bax、Bc l-2 蛋白表达。结果处理48 h后,随着冬凌草甲素浓度(1050 mol/L)的增加,U266细胞、RPMI8226细胞的细胞增殖抑制率、细胞凋亡率均显著升高,差异均有统计学意义(P*通讯作者:祝龙,E-mail:文章编号:16 7 1-46 9 5(2 0 2 3)16-16 9 2-0 4临床和实验医学杂志2 0 2 3年8 月第2 2 卷第16 期0.05)。处理48 h后,随着冬凌草甲素浓度(10 50 umol/L)的升高,促凋亡蛋白Bax表达量逐渐升高,而抗凋亡蛋白Bcl-2表达量逐渐降低,差异均有统计学意义(P0.05)。结论冬凌草甲素具有抑制MM细胞增
14、殖和诱导细胞凋亡的潜力,其作用机制可能与促进Bax表达,抑制Bcl-2表达,改变细胞Bcl-2/Bax比例,调节细胞凋亡有关。【关键词】多发性骨髓瘤细胞增殖细胞凋亡冬凌草甲素Experimental study on the proliferation and apoptosis of multiple myeloma U266 cells,RPMI8226 cells induced by oridonin.ZHAN Qi-lin,ZHU Long,JIN Wei-yun,et al.1 Department of Hematology,Shanghai Public Health Clin
15、ical Center,Shanghai 200093,China;2 De-partment of Hematology Oncology,Jinshan Branch,Shanghai Sixth Peoples Hospital,Shanghai 201599,China.Abstract)Objective To investigate the effect of dongling grass methylene on the proliferation and apoptosis of multiple myeloma(MM)U266 cells,RPMI8226 cells.Met
16、hods The cell culture and treatment methods was used,MM related U266 cells and RPMI8226 cells were trea-ted with 0,10,20,30,40,and 50 mol/L oridonin for 48 hours.The proliferation of U266 cells and RPMI8226 cells was measured by MTTmethod,the apoptosis of U266 cells and RPMI8226 cells was detected b
17、y Annexin V-FITC/PI staining kit,and the expression of Bax and Bcl-2 proteins was determined by Western blotting.Results After 48 hours of intervention,as the concentration of oridonin increases,the cell prolif-eration inhibition rate and apoptosis rate of U266 cells and RPMI8226 cells were signific
18、antly increased,and the dfferences were statistically sig-nificant(P 0.05).After 48 hours of intervention,as the concentration of oridonin A increased,the expression of pro apoptotic protein Baxgradually were significantly increased while the expression of anti apoptotic protein Bcl2 gradually were
19、significantly decreased,and the dffer-ences were statistically significant(P 0.05).Conclusion The results of this study indicate that dongling grass methylene has the potential toinhibit proliferation and induce apoptosis in multiple myeloma cells.This provides a scientific basis for the use of dong
20、ling grass methylin as a can-didate drug for the treatment of multiple myeloma.Key words】M u l t i p l e my e l o ma;Ce l l p r o l i f e r a t i o n;A p o p t o s i s;O r i d o n i n A多发性骨髓瘤(multiple myeloma,M M)是一种恶性血液肿瘤,主要发生在骨髓中的浆细胞。MM的患病率逐年增加,给患者的生活质量和预后带来了巨大的挑战。目前,尽管有联合化疗、造血干细胞移植及新的靶向药物治疗等方法,但仍
21、然无法根治,MM的治疗仍然有很大的复杂性和难度。因此,探索新的治疗策略对于改善MM患者的预后至关重要。冬凌草甲素是一种天然的二枯类化合物,被广泛用于中药中,具有广泛的药理活性。近年来,冬凌草甲素在肿瘤治疗中的应用引起了研究人员的兴趣。冬凌草甲素可通过调节多种信号通路和靶点的活性,对肿瘤细胞的生存和增殖产生抑制作用,显示出潜在的抗肿瘤效果2-3。本研究旨在探讨冬凌草甲素对MM相关的U266细胞、RPMI8226细胞增殖和调亡的影响,了解其抗肿瘤活性,为其在MM治疗中的潜在应用提供实验依据。现将结果报道如下。1材料与方法1.1材料与试剂1.1.1乡细胞系MM相关的U266细胞、RPMI8226细胞
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